CD53 anticorps

N° du produit ABIN118730

L’anticorps Souris Monoclonal anti-CD53 a été validé pour FACS, WB, IP, Func et IHC (fro). Il convient pour détecter CD53 dans des échantillons de Humain.

Aperçu rapide pour CD53 anticorps (ABIN118730)

Antigène: CD53

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD53 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MEM-53

Détail du produit anti-CD53 anticorps

Specificité: The antibody reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes, it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).

Purification: Protein-A affinity chromatography

Pureté: > 95 % pure (by SDS-PAGE)

Immunogène: Leukocytes of pacient suffering from a LGL-type leukemia

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry. Immunoprecipitation. Western Blotting. Immunohistochemistry on frozen sections. Suitable for discrimination of lymphomas from other tumors. Functional Application. The antibody induces activation of monocytes and B lymphocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CD53

Antigène: CD53

Autre désignation: CD53

Sujet: CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.Synonyms: Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, MOX44, TSPAN25, Tetraspanin-25

ID gène: 963

NCBI Accession: NP_000551

UniProt: P19397